m. hominis-specific medium (SAS institute)
Structured Review

M. Hominis Specific Medium, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m%2E+hominis-specific+medium/pmc07669805-55-29-36?v=SAS+institute
Average 90 stars, based on 1 article reviews
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1) Product Images from "MYCO WELL D-ONE detection of Ureaplasma spp. and Mycoplasma hominis in sexual health patients in Wales"
Article Title: MYCO WELL D-ONE detection of Ureaplasma spp. and Mycoplasma hominis in sexual health patients in Wales
Journal: European Journal of Clinical Microbiology & Infectious Diseases
doi: 10.1007/s10096-020-03993-7
Figure Legend Snippet: Sensitivity, specificity, positive predictive value, negative predictive value and accuracy for the MYCO WELL D-ONE detection of Mycoplasma hominis
Techniques Used:
Figure Legend Snippet: Flow chart displaying CLSI-compliant AST of M . hominis presumptively identified as resistant by MYCO WELL D-ONE. The MYCO WELL D-ONE over-reported clindamycin resistance ( p = 0.05; Fisher’s exact test). One further tetracycline-resistant M . hominis isolate was found although it was missed by the initial assay screening
Techniques Used:
Figure Legend Snippet: Nucleotide alignment of the tet (M) gene found in the U . parvum and M . hominis strains, both co-incidentally isolated from the same patient. Shown here are nineteen of the twenty-four mismatches in nucleotide identity were clustered in a 180 nucleotide stretch in the middle of the 1920 nucleotide open reading frame, confirming different origins for these genes and not transfer between these two co-infecting species
Techniques Used: Isolation
Figure Legend Snippet: Bacterial loads for Ureaplasma spp. ( a ) or M . hominis ( b ) in swab or urine samples. Bacterial loads from urine samples were more frequently amenable for AST, based on CLSI guidelines, for < 10 5 CCU/ml (black fraction of the bar). a For Ureaplasma -positive urine samples 5.9% possessed a load > 10 5 CCU/mL (grey fraction of the bar), whereas for Ureaplasma -positive swab samples, 55.9% possessed a load > 10 5 CCU/mL. The prevalence of a Ureaplasma load of > 10 5 CCU/mL captured on swab samples was significantly higher than that of urine samples, for Ureaplasma positive patients ( p ≤ 0.0001; Fisher’s exact test). b For M . hominis -positive urine samples, 7% possessed a load > 10 5 CCU/mL. Whereas for M . hominis -positive swab samples, 40% possessed a load > 10 5 CCU/mL. The prevalence of a M . hominis load of > 10 5 CCU/mL captured on swab samples was significantly higher than that of urine samples, for M . hominis positive patients ( p = 0.0004; Fisher’s exact test)
Techniques Used:
Figure Legend Snippet: Bacterial load comparison for matched Ureaplasma -positive ( a ) or M. hominis -positive ( b ) patient samples. a All paired Ureaplasma -positive samples possessed bacterial loads equal to or greater their urine counterparts. Statistical analysis revealed swabs to possess significantly greater Ureaplasma loads than urines ( p = 0.0255; paired t test). b 76.2% of paired M . hominis -positive samples possessed bacterial loads equal to or greater their urine counterparts. However, 2 urine samples detected M . hominis where swab samples did not and 2 swab samples detected M . hominis in patients with negative culture results from urine. A universal trend of lower bacterial load in urine compared to matched swabs was not always seen for this organism. Statistical analysis of matched M . hominis -positive samples failed to achieve significance ( p = 0.0939; paired t test)
Techniques Used: Comparison
Figure Legend Snippet: Bar and whisker graphs displaying the min-max detection times for Ureaplasma spp. and M . hominis . Samples from males and females are examined separately comparing the MYCO WELL D-ONE assay to culture titration assay in specialist media. Mean values are represented by + symbol. Time to detection of Ureaplasma spp. in female samples was 28.14 h (culture titration) and 27.57 (MYCO WELL D-ONE) ( a ) and 34.09 h in males was for both assays ( b ). Time to detection of M . hominis in females was 43.26 and 43 h for MYCO WELL D-ONE and culture titration, respectively ( c ), and 46.25 h in male samples ( d ). There was no significant difference in time to detection between assays
Techniques Used: Whisker Assay, Titration
Figure Legend Snippet: Details of resistant isolates
Techniques Used: Mutagenesis